HPLC ANALYSIS OF PHENOLIC COMPOUNDS IN NORWAY SPRUCE WITH PHOTODIODE ARRAY AND MASSSPECTROMETRIC DETECTION

Abstract

This paper deals with phenolic compounds in Norway spruce needles and practical
method for their determination. The importance of phenolic compounds in plants consists in
their ability to absorb radiation just in the UV-B range and thus protect plants from its harmful
effect. Identification of phenolic compounds according to their spectral properties was carried
out by liquid chromatography. For quantification of phenolic compounds photometric signals at
selected wavelengths measured on HPLC system Dionex with Chromeleon software were
chosen. Three procedures were used in this study to isolate UV-light screening substances from
Norway Spruce needles. In extracts from Norway spruce needles besides phydroxyacetophenone as major component and catechin, six phenolic acids were identified.
However, in order to simplify chromatograms and eliminate interfering components, sample
fractionation based on ion-exclusion or hydrophilic interactions is promising and will be a
subject of further study